FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63136450
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCG72150.013.9689829
CGCCGTA90700.011.15908245-49
ATCGGGA71850.09.6090041
GAGCGGC105000.09.5988088
GCGTCGG37900.08.799141145
GTCGCCG121450.08.59542740-44
GCCGTAT123200.08.50957645-49
AGCGTCG284700.08.5063919
GAGCGTC339350.07.96979769
AGAGCGG137150.07.61306527
TGGTCGC135800.07.54835140-44
CCGTATC144450.07.29788845-49
GGTCGCC145450.07.256882740-44
AGAGCGT385500.07.09102158
CGTATCA152650.07.00085445-49
ATCGGAA446400.06.7562461
GTGGTCG154450.06.69320440-44
AAGAGCG456350.06.68856866
TCGGAAG452000.06.61196572
GATCGGA412550.06.42980581
Produced by FastQC (version 0.11.5)