FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64988593
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT972240.14960163855216868TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG90050.043.0974352
CACCGGT90350.042.9407351
CCGGTGG166450.023.3931273
CGGTGGT189000.020.8275554
GATCGGA887800.018.7498191
ATCGGAA909550.018.3514922
TCGGAAG910650.018.2738083
CGGAAGA927400.017.9475464
TATGCCG257150.016.02972445-49
ACGACGC52100.014.58231825-29
CGTATGC284150.014.31267945-49
CGACGCT54450.014.27249425-29
TCTCGTA276100.014.24142740-44
AGAGCAC1172950.014.1655918
CTCGTAT283950.014.14386740-44
GAGCACA1221650.013.6958379
ATGCCGT294800.013.62839745-49
GCCGTCT300900.013.376950-54
GAAGAGC1290400.013.1571796
ATCTCGT317800.012.31799940-44
Produced by FastQC (version 0.11.5)