FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71812018
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC559700.013.7538699
AGAGCGT616900.012.5960718
GATCGGA686800.011.6436091
ATCGGAA706450.011.3917472
TCGGAAG720550.011.147373
CGCCGTA170250.011.08842155-59
AAGAGCG741950.010.7759597
CGGAAGA760050.010.653024
TCGCCGT192350.09.91241455-59
GCCGTAT201450.09.47184355-59
CGTATCA226250.08.37588460-64
GTCGCCG226600.08.30540255-59
TGGTCGC243650.08.02163350-54
GTGGTCG260500.07.58625550-54
CGGTGTA265350.07.33248135-39
GAAGAGC1201900.07.08045966
CCGTATC222300.06.881081660-64
GCGTCGG76150.06.476382145
ACCGGTG301950.06.424489535-39
TCTCGGT324750.06.290670445-49
Produced by FastQC (version 0.11.5)