FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64172654
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7967301.2415412957675087Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC1162400.0111.3731469
AGAGCGT1212500.0107.102538
GATCGGA1226550.0106.275331
ATCGGAA1254300.0104.3107452
TCGGAAG1260800.0103.611983
AAGAGCG1281550.0102.209177
CGGAAGA1288900.0101.4352344
GAAGAGC1725950.076.089036
GGAAGAG2250250.058.6789175
CGCCGTA943400.026.0368245-49
TCGCCGT955150.025.31427445-49
GCCGTAT975650.025.28019745-49
GTCGCCG976750.025.2082840-44
TGGTCGC993900.024.89146640-44
CCGTATC995350.024.73906345-49
GGTCGCC1002150.024.72706240-44
GTGGTCG1012400.024.50106240-44
CGTATCA1007200.024.31125545-49
GCGTCGT1089500.023.85713210-14
CGTCGTG1090900.023.84114310-14
Produced by FastQC (version 0.11.5)