FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54823123
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA70300.013.73588755-59
TCGCCGT84600.011.32840855-59
GTCGCCG93750.010.640326555-59
GCCGTAT100550.09.94958155-59
GAGCGTC370350.09.6892769
TGGTCGC112500.08.98292650-54
AGAGCGT410450.08.8486278
CGTATCA122200.08.246448560-64
GCGTCGG38850.08.21044145
GTGGTCG127450.08.04298350-54
AAGAGCG476550.07.92551857
GATCGGA473150.07.7908321
ATCGGAA489000.07.62113572
TCGGAAG491500.07.55213643
CGGAAGA505200.07.41866254
GGTCGCC117150.06.59663955-59
CCGTATC113950.06.527642760-64
TCTCGGT165800.06.506216545-49
CGATCGG15258.847888E-46.1798553145
AGATCGG485100.05.977692145
Produced by FastQC (version 0.11.5)