FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61565212
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA111400.017.08655255-59
GCGTCGG51950.016.603914145
TCGCCGT129900.014.4522555-59
GTCGCCG144300.013.18081755-59
GCCGTAT148500.013.12041755-59
TGGTCGC166200.012.06347550-54
GTGGTCG176850.011.41078350-54
CGTATCA171500.010.8875860-64
AGCGGCG86500.010.7268259
CGGTGTA200500.09.83366435-39
AGCGTCG495750.09.62144859
TCTCGGT229600.09.31344845-49
ACCGGTG223550.08.72244935-39
GAGCGTC569150.08.5079919
CTCGGTG257300.08.46855945-49
CCGGTGT247600.08.1504135-39
CACCGGT229650.08.13724335-39
AGAGCGT618300.07.92546468
CGGTGGT252100.07.77472650-54
AAGAGCG714100.07.4607236
Produced by FastQC (version 0.11.5)