FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68699523
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA435050.022.0937791
TCGGAAG449800.021.4418432
CGGAAGA475400.020.3027973
TATGCCG177450.014.99453645-49
CTCGTAT192800.014.1465140-44
CGTATGC198950.013.63636540-44
TCTCGTA183200.013.38479240-44
ATGCCGT219350.012.34171845-49
TGCCGTC219300.012.33792345-49
TCGTATG223700.012.30256740-44
GCCGTCT221900.012.16070445-49
AGAGCAC838900.011.8851747
AGCACAC880600.011.3307729
GAGCACA893950.011.2101968
GATCGGA350900.010.8609591
GAAGAGC930100.010.7976895
CGTCTTC267200.010.12603650-54
CGTCTGA333950.08.18866315-19
AAGAGCA1285850.08.1202796
ATCTCGT220100.07.95438835-39
Produced by FastQC (version 0.11.5)