FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67760843
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT1312360.19367527644247284TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA454650.048.8805051
ATCGGAA501450.044.4419332
TCGGAAG517000.042.7666663
CGGAAGA544800.040.5311364
AGAGCAC911550.024.382158
GAGCACA989600.022.3566049
GAAGAGC1004350.022.316676
TATGCCG271350.019.7097445-49
TCTCGTA273300.018.31696540-44
CGTATGC290950.018.2126145-49
CTCGTAT288150.018.11237140-44
CTTACGG310650.017.10419530-34
AAGAGCA1340200.016.9350577
ATGCCGT306800.016.92683645-49
TTACGGT308750.016.70246330-34
GCCGTCT323950.016.13435250-54
ACTTACG329450.016.08415430-34
ATCTCGT314200.015.90954840-44
CGGTGAT312350.015.76739535-39
GGAAGAG1570950.014.6596795
Produced by FastQC (version 0.11.5)