FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49065199
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA422750.012.04719451
TATGCCG129600.011.75865245-49
ATCGGAA444700.011.5625292
TCGGAAG449350.011.4393073
CGGAAGA471600.010.9141524
CTCGTAT144700.010.3613140-44
TCTCGTA141450.010.16883940-44
CGTATGC147100.010.05419545-49
ATGCCGT161000.09.41131345-49
GCCGTCT167200.08.9584350-54
ATCTCGT173000.08.28082340-44
CGTCTTC204600.07.809870750-54
AGAGCAC705550.07.56216578
CCGTCTT204500.07.537161450-54
GAGCACA748550.07.32145079
GAAGAGC782300.06.8480566
TCGTATG164950.06.540040545-49
GTATGCC254300.06.391739445-49
TGCCGTC169800.06.378973550-54
AGATCGG420350.05.6412735145
Produced by FastQC (version 0.11.5)