FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28098318
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC193600.020.0334139
AGAGCGT213800.018.2762728
CGCCGTA52450.017.1939255-59
GATCGGA228750.017.1706961
ATCGGAA241550.016.4196872
TCGGAAG243850.016.2915923
AAGAGCG245500.016.0935387
CGGAAGA255050.015.5192794
TCGCCGT62050.014.46368355-59
GCCGTAT66100.013.86263155-59
GTCGCCG66950.013.6866355-59
TGGTCGC75700.012.18135250-54
CGTATCA80300.011.68206260-64
GTGGTCG79550.011.62825950-54
CCGTATC74700.010.44221160-64
GGTCGCC78200.010.08607555-59
GAAGAGC435700.09.61712556
TCTCGGT108350.09.04575945-49
CGGTGGT112450.08.58714950-54
CTCGGTG114300.08.4860845-49
Produced by FastQC (version 0.11.5)