FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59873223
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA55950.014.22764155-59
TCGCCGT75000.010.74915155-59
GAGCGTC332500.09.9426879
GTCGCCG84400.09.77530855-59
GCGTCGG38250.09.28923145
AGAGCGT367550.09.2507318
GCCGTAT89100.09.16202255-59
CGACCGG24800.08.7710512
TGGTCGC102600.08.38012150-54
GCGACCG25700.08.1842181
AAGAGCG430100.08.09087
GATCGGA415300.08.0684921
ATCGGAA431350.07.88360932
TCGGAAG439250.07.7417053
GTGGTCG114050.07.615080450-54
CGGAAGA455400.07.5783434
CGTATCA114550.07.29091160-64
CCGTATC105300.06.5819460-64
GGTCGCC107650.06.33059455-59
AGCGTCG271050.06.1798463145
Produced by FastQC (version 0.11.5)