FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52618731
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTTATATCTCGTAT960890.1826136780075521TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA521750.032.9348831
ATCGGAA556850.031.0136642
TCGGAAG566400.030.3954473
CGGAAGA583950.029.4819434
AGAGCAC826550.020.8550348
GAGCACA871900.019.8617559
GAAGAGC918600.019.0966456
TATGCCG229150.018.96237845-49
CTCGTAT249800.017.0638240-44
CGTATGC251900.016.8066345-49
TCTCGTA251900.016.77767640-44
ATGCCGT263250.016.28028345-49
TATCTCG263050.015.54841640-44
GCCGTCT278900.015.15986150-54
AAGAGCA1166500.015.1315157
ATCTCGT282550.014.67547140-44
CGTCTTC307950.014.10174450-54
CCGTCTT312850.013.72793150-54
GGAAGAG1338450.013.4854475
CGTGCTT326550.013.27055930-34
Produced by FastQC (version 0.11.5)