FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56669133
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA290000.019.540011
ATCGGAA313000.018.3193722
TCGGAAG337200.016.897153
CGGAAGA355200.016.1633224
TATGCCG115200.012.82533545-49
CTCGTAT127200.011.51247340-44
CGTATGC132450.010.94699945-49
TCTCGTA132150.010.82889940-44
ATGCCGT148800.09.93903545-49
TATCTCG144250.09.82003640-44
AGAGCAC646000.09.2699828
ATCTCGT164150.08.81503440-44
GAGCACA699200.08.57502659
GCCGTCT171100.08.53429650-54
GAAGAGC741800.08.081616
TCGTATG149000.08.02813445-49
AGTCACG199850.07.718891625-29
CGTGCTT198850.07.67791430-34
GTCACGT197900.07.67040725-29
CGTCTTC203350.07.644319550-54
Produced by FastQC (version 0.11.5)