FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56669133
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC293700.018.8076179
GATCGGA324600.017.3672221
AGAGCGT328850.017.1059728
ATCGGAA349200.016.3393422
TCGGAAG376950.015.2312553
CGGAAGA401050.014.2255954
CGCCGTA102000.013.97480455-59
AAGAGCG411000.013.8635787
TCGCCGT120350.011.96452755-59
GCCGTAT130500.011.078455-59
GTCGCCG138200.010.2932755-59
CGTATCA149450.09.63355660-64
TGGTCGC154200.09.44029850-54
GTGGTCG167950.08.81418550-54
AGGTCGT167750.08.565577535-39
CCGTATC144200.07.75215560-64
GAAGAGC781600.07.6425326
GGTCGTG194550.07.639028535-39
TCTCGGT213200.07.249398745-49
GGTCGCC164700.06.849807755-59
Produced by FastQC (version 0.11.5)