FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63993515
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC263400.023.5580399
GATCGGA289600.021.7762411
AGAGCGT298850.021.0061178
ATCGGAA313550.020.1405142
TCGGAAG342050.018.3955153
CGGAAGA371700.017.1231144
AAGAGCG376150.016.727897
CGCCGTA110650.011.71412155-59
TCGCCGT125800.010.38407455-59
GCCGTAT143500.09.18407955-59
GTCGCCG144250.09.12627855-59
TGGTCGC160500.08.30157150-54
GAAGAGC820100.08.1409476
CCGTATC162050.07.945049360-64
CGTATCA171850.07.880068360-64
GGTCGCC167300.07.712899755-59
GTGGTCG176250.07.65021550-54
GCGTCGT192350.06.69319810-14
CGTCGTG196500.06.57397510-14
AGCGTCG208450.06.00235610-14
Produced by FastQC (version 0.11.5)