FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66867404
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA189350.022.72021
ATCGGAA214300.020.5000952
TCGGAAG234800.018.4631163
CGGAAGA260600.016.7736244
TATGCCG100550.010.10823845-49
CTCGTAT121100.08.94413740-44
TCTCGTA120250.08.16329640-44
ATGCCGT140700.07.625654745-49
CGTATGC124300.07.61695845-49
AGAGCAC655050.07.28183138
TCGTATG134150.06.93879245-49
GAGCACA719550.06.53842169
GAAGAGC747900.06.4067786
TGCCGTC148050.06.228733550-54
ATCTCGT154500.06.12839340-44
GCCGTCT155250.06.098634750-54
CGTCTTC194700.05.51082750-54
CCGTCTT196300.05.118750650-54
ACACGTC202100.05.114944510-14
Produced by FastQC (version 0.11.5)