FastQCFastQC Report
Wed 15 May 2024
EGAF00005004898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269807478
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGTGC5825680.2159198864013695No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA4616860.17111682871888378No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG4437160.16445652407010009No Hit
GATCGGAAGAGCACACGTCTGAACTCCA3973220.14726130014824867Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTCGTTATCGGAATTAACCAGACAAA3357790.12445133192342431No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2870930.10640661338526725No Hit
CCACCTACTAAGAACGGCCATGCACCAC2789250.10337926956939274No Hit
GCAGCCATACTCCCCCCGGAACCCAAAG2719650.10079965240993059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)