FastQCFastQC Report
Wed 1 May 2024
EGAF00005004906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226297744
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT7509398933.183710837170345No Hit
CTATAGTG5442858924.051759437778575Clontech Universal Primer Mix Short (100% over 8bp)
AGTCCCAC5432445124.005741303368893No Hit
TACGTTCA3995002017.65374205409666No Hit
CTATAGTA3303900.1459979203327807No Hit
AGTCCCAA2341240.10345838887373089No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)