FastQCFastQC Report
Wed 1 May 2024
EGAF00005004915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263716125
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC10222227438.76223875199137No Hit
GAGGATCT6222215123.594367238635865No Hit
CTTATGAG4933706718.70839979921592No Hit
AGACCATA3238271912.279385267017707No Hit
CTTAGGAG126831824.809407084985796No Hit
CTTCTGAG10478330.39733368598526164No Hit
TCCTGCGA3954810.14996466370799283No Hit
AGATCATA3637900.1379475752572961No Hit
TCCAGCGC2835720.10752926086715403No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)