FastQCFastQC Report
Tue 5 Jul 2022
EGAF00005004924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240570356
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43801581.8207388777360416Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6267230.2605154726544945Clontech SMART CDS Primer II A (100% over 24bp)
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC5929680.24648423432519673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5752260.23910926082679945No Hit
GTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTC5262280.21874183035253103No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG5225850.21722751243715166No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA4428290.18407463303583424No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG4401120.18294523370119634No Hit
GGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCA4374310.1818307988038227No Hit
CAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCC3856270.16029697358056866No Hit
GGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCG3815880.15861804685528255No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC3646780.15158891812921457No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC3574720.14859353660348742No Hit
GGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCG3501790.14556199102103834No Hit
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACC3409720.1417348361907067No Hit
CTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGG3354400.13943530099776716No Hit
GATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGC3236370.13452904396915802No Hit
GTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGA3149000.13089725818088743No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT3146090.13077629564633475No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCG3140960.1305630524153192No Hit
CCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGC3059330.1271698662656508No Hit
GTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTC3046960.12665567157409868No Hit
GGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTCATCCAC3015500.12534794602872848No Hit
GTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGT2998960.12466041327219884No Hit
GCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGG2988570.12422852298560011No Hit
GGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGC2970810.12349027741389716No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA2882160.1198052847375759No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC2838910.11800747387180158No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2825690.1174579464811533Clontech SMART CDS Primer II A (100% over 25bp)
GTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCA2763840.11488697302339279No Hit
GTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGAC2624950.10911360999108302No Hit
GCCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCAC2595440.10788694181422752No Hit
CGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTC2591380.10771817621619181No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC2540790.10561525710175199No Hit
CCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCAC2415810.10042010329818025No Hit
GGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAG2413990.10034444975423322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)