FastQCFastQC Report
Mon 13 May 2024
EGAF00005004926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256617222
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCA4732730.1844276063435836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGCAGGCTCCACTCCTGGTGGTGC3240860.12629160173824966No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA3045350.11867286132495036No Hit
CGTTCGTTATCGGAATTAACCAGACAAA2739770.10676485306196634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)