FastQCFastQC Report
Mon 13 May 2024
EGAF00005004930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241266221
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGA6333380.2625058731284227No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA5734790.23769552058429266No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC4698440.19474089578416365No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG4590030.19024751915022534No Hit
GATCGGAAGAGCACACGTCTGAACTCCA3743350.15515433467994677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCTACTAAGAACGGCCATGCACCAC2752060.1140673563250282No Hit
AGGTCCGTCTTGCGCCGGTCCAAGAATT2626930.10888096929242323No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT2541220.10532846203944977No Hit
TGACTTTTACTTCCTCTAGATAGTCAAG2513190.10416667486991475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)