FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66290030
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC593500.015.1039999
CGCCGTA140150.014.8088555-59
AGAGCGT644500.014.0661458
TCGCCGT158900.012.96109355-59
GATCGGA727000.012.6601471
AAGAGCG739900.012.4581847
ATCGGAA741850.012.4486172
TCGGAAG749350.012.2919873
CGGAAGA761250.012.1473094
GCCGTAT172700.012.14360555-59
GTCGCCG181000.011.55471455-59
CGTATCA201000.010.51319360-64
TGGTCGC203250.010.33277650-54
GTGGTCG218250.09.80192350-54
CCGTATC190450.09.40612360-64
GGTCGCC212750.08.48828455-59
GAAGAGC1193300.08.1864766
TCTCGGT277200.07.91610545-49
GCGTCGG55150.07.3585124145
CGGTGGT304450.07.25041950-54
Produced by FastQC (version 0.11.5)