FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42458221
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTCTCATCTCGTAT534140.1258036694471961TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA562350.016.9210741
TCGGAAG570300.016.8164443
ATCGGAA573700.016.7158012
TATGCCG143900.016.67695245-49
CGGAAGA581000.016.3195924
CTCGTAT163450.014.37034640-44
CGTATGC162350.014.1654545-49
ATGCCGT169250.013.97348745-49
TCTCGTA165450.013.80228340-44
GCCGTCT173100.013.4027250-54
AGAGCAC764050.012.599468
GAGCACA795650.012.2265839
CGTCTTC198450.012.20943250-54
CCGTCTT203050.011.56863450-54
GAAGAGC848000.011.4205296
ATCTCGT195300.011.40318340-44
TGCCGTC170650.011.34344750-54
TCGTATG173050.011.1863745-49
CATCTCG220200.010.10713840-44
GTATGCC253950.09.48991445-49
Produced by FastQC (version 0.11.5)