FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42254660
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC479400.020.7480859
CGCCGTA111950.020.22238255-59
AGAGCGT516150.019.3815618
TCGCCGT122450.018.4293355-59
GATCGGA568400.018.079781
AAGAGCG579100.017.3625667
GTCGCCG133700.017.25682355-59
GCCGTAT133200.017.08298355-59
ATCGGAA608500.016.8571152
TCGGAAG612800.016.6791613
CGGAAGA614950.016.5854514
TGGTCGC146700.015.65848150-54
CGTATCA148950.015.2759360-64
GTGGTCG155400.014.99567450-54
CCGTATC146650.013.51573560-64
GGTCGCC154900.012.78713955-59
GCGTCGG30350.012.139402145
TCTCGGT197050.012.038815545-49
GAAGAGC872450.011.9551476
CTCGGTG211850.011.2727945-49
Produced by FastQC (version 0.11.5)