FastQCFastQC Report
Mon 13 May 2024
EGAF00005004950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252838654
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGTGC6028710.23844099407363561No Hit
CGTTCGTTATCGGAATTAACCAGACAAA5058100.20005248089953842No Hit
GATCGGAAGAGCACACGTCTGAACTCCA3710980.14677265288716496Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTCGTTCGTTATCGGAATTAACCAGA3598780.14233504027434035No Hit
CCACCTACTAAGAACGGCCATGCACCAC3271380.1293860708497523No Hit
CGTTGGGTTGACAGTGAGGGTAATAATG3021040.1194848949006033No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2752620.10886863841633962No Hit
GCACTCTCGACTGCCGGCGACGGCCGGG2531740.10013263240991625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)