FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19060855
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA25550.011.40620255-59
GAGCGTC115350.010.6836799
TCGCCGT30100.09.87468555-59
AGAGCGT128000.09.62783058
GATCGGA138600.09.0452171
GCCGTAT35400.08.76488955-59
ATCGGAA142800.08.6315732
TCGGAAG148650.08.3879833
AAGAGCG150250.08.1538317
CGGAAGA154700.08.15366654
GTCGCCG37800.07.939889455-59
TGGTCGC42000.07.42196550-54
CGTATCA43650.07.041861560-64
GTGGTCG47150.06.642044550-54
CCGTATC42150.05.847728760-64
GGTCGCC45950.05.648113755-59
TCTCGGT62050.05.491156645-49
CGTCGTG90850.05.346324145
TAGATCG53550.05.2797165145
GCGTCGT89550.05.10012145
Produced by FastQC (version 0.11.5)