FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53680446
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT13464262.5082243169142076TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1834900.0126.2811661
ATCGGAA1875350.0123.5963752
TCGGAAG1873400.0122.946513
CGGAAGA1921250.0119.420814
AGAGCAC2207700.0102.652578
GAAGAGC2271050.0100.353346
GAGCACA2261250.0100.01689
AAGAGCA2575850.088.434187
GGAAGAG2705350.084.822025
TATGCCG1719100.027.90102645-49
TCTCGTA1675500.027.55840340-44
CTTACGG1703800.027.41470130-34
CGTATGC1731250.027.31244345-49
GCCGTCT1740050.026.92739550-54
CGGTGAT1655700.026.83079735-39
ACTTACG1739400.026.83027330-34
CGTCTTC1769450.026.82741750-54
ATCTCGT1702800.026.7819640-44
CTCGTAT1731600.026.57096140-44
CCGTCTT1773900.026.49284250-54
Produced by FastQC (version 0.11.5)