FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45173177
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTACATGTGTAGATCT2617410.5794168517303975Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC435100.094.427399
GATCGGA442100.093.003851
AGAGCGT460500.089.360698
ATCGGAA464900.088.921192
TCGGAAG476450.086.993043
CGGAAGA505100.082.144784
AAGAGCG513450.080.385557
GAAGAGC831450.050.0854536
GGAAGAG1183100.035.4682545
CGCCGTA326500.025.543555-59
TCGCCGT335400.024.85272255-59
GTCGCCG349100.023.9438655-59
GCCGTAT349400.023.73656355-59
TGGTCGC363250.022.94246150-54
CGTATCA369700.022.5814760-64
GTGGTCG374600.022.38666350-54
CCGTATC362700.022.3537160-64
GGTCGCC366900.022.19736555-59
GCGTCGT382550.021.61240610-14
CGTCGTG384950.021.43623710-14
Produced by FastQC (version 0.11.5)