FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59762737
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTACATGTGTAGATCT4332520.7249534103499978Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC651000.0102.088959
GATCGGA670150.099.705471
ATCGGAA691850.096.6746442
AGAGCGT689150.096.542548
TCGGAAG707200.094.538233
CGGAAGA751950.088.9217154
AAGAGCG764350.087.167567
GAAGAGC1171900.057.2431266
GGAAGAG1678050.040.382975
CGCCGTA508150.026.4772955-59
TCGCCGT519750.025.87798755-59
GTCGCCG539700.025.01275455-59
GCCGTAT539400.024.84387255-59
TGGTCGC553800.024.22050750-54
GTGGTCG565650.023.87715350-54
CGTATCA566350.023.77932560-64
CCGTATC555750.023.53111860-64
GGTCGCC562600.023.30392355-59
GCGTCGT583500.022.94646610-14
CGTCGTG587900.022.78705810-14
Produced by FastQC (version 0.11.5)