FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105762384
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC759650.012.7681659
AGAGCGT840800.011.7168978
CGCCGTA215900.011.15426755-59
GATCGGA890000.010.8618491
ATCGGAA926850.010.6904522
TCGGAAG954200.010.3840353
AAGAGCG991550.09.9428057
CGGAAGA1013600.09.8114444
TCGCCGT248450.09.72793455-59
GCCGTAT261750.09.355555-59
GTCGCCG283850.08.519834555-59
CGTATCA305400.08.19867260-64
TGGTCGC315250.07.79084150-54
GTGGTCG336300.07.380790750-54
CCGTATC299500.06.72396860-64
GAAGAGC1704700.06.19565446
TCTCGGT422700.06.150032545-49
GGTCGCC330350.06.122431355-59
CGGTGGT475650.05.45621650-54
CTCGGTG476450.05.431880545-49
Produced by FastQC (version 0.11.5)