FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66694279
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG110200.010.96831545-49
GATCGGA401900.010.7574021
ATCGGAA434400.010.0571692
TCGGAAG434550.010.0155563
CGTATGC122350.09.55519945-49
CGGAAGA458800.09.470114
CTCGTAT131500.09.0836140-44
TCTCGTA129350.08.81881840-44
ATGCCGT148750.08.30655445-49
GCCGTCT156000.07.566140750-54
ATCTCGT167250.06.87118540-44
CGTCTTC199300.06.37678850-54
TAGATCG152450.06.360718145
AGATCGG403250.06.353999145
TCGTATG147650.06.230796345-49
CCGTCTT204800.05.984404650-54
TGCCGTC149600.05.95994150-54
AGAGCAC794700.05.74312548
GAGCACA858150.05.59884459
GAAGAGC897800.05.2885676
Produced by FastQC (version 0.11.5)