FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70659547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA445400.019.857541
ATCGGAA471950.018.9272442
TCGGAAG490600.018.158793
CGGAAGA512600.017.3653034
TATGCCG154250.012.99953845-49
CGTATGC173500.011.36502445-49
TCTCGTA176750.011.04930240-44
CTCGTAT180050.010.991734540-44
AGAGCAC886700.010.3821828
TATCTCG188400.010.24292140-44
ATGCCGT196050.010.1539445-49
GAGCACA961350.09.6815559
GCCGTCT211800.09.501842550-54
GAAGAGC993650.09.3522736
TCGTATG199300.09.19540145-49
ATCTCGT220750.09.011147540-44
TGCCGTC208100.08.8207650-54
CGTCTTC251200.08.27124550-54
CCGTCTT258300.07.92039750-54
CGTGCTT274200.07.581851530-34
Produced by FastQC (version 0.11.5)