FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74949478
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACCACCGGTGTAGATCT2096250.27968840556834834Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC771350.046.1596539
AGAGCGT831150.042.9955148
GATCGGA931800.039.4415781
ATCGGAA953500.038.492612
TCGGAAG967950.037.816583
AAGAGCG955100.037.780217
CGGAAGA988100.036.9798434
GAAGAGC1451800.025.4486456
CGCCGTA349100.022.22660855-59
TCGCCGT368650.021.0754355-59
GCCGTAT382850.020.25208355-59
GTCGCCG396350.019.53301655-59
TGGTCGC417700.018.62513750-54
CGTATCA419900.018.45523860-64
GTGGTCG431250.018.19457850-54
GGAAGAG2077150.018.1112655
CGGTGTA439350.017.64887435-39
ACCGGTG462250.016.74945335-39
CCGTATC409550.016.5958160-64
CACCGGT465750.016.44925335-39
Produced by FastQC (version 0.11.5)