FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16951567
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC131250.011.5429169
CGCCGTA38800.010.68719855-59
AGAGCGT146500.010.3907998
TCGCCGT43050.09.598453555-59
GATCGGA164400.09.3943141
ATCGGAA168200.09.2248132
GCCGTAT45450.09.09160555-59
TCGGAAG171100.09.0661593
CGGAAGA178050.08.9565414
AAGAGCG173650.08.849677
GTCGCCG49000.08.37374955-59
CGTATCA53250.08.11363960-64
TGGTCGC52650.07.90333350-54
GTGGTCG57100.07.490532450-54
GCGTCGG17551.582672E-56.6114383145
CCGTATC50550.06.22382960-64
TCTCGGT74550.06.06748745-49
GAAGAGC280800.05.9631156
GGTCGCC56150.05.73235155-59
AGATCGG168550.05.5932946145
Produced by FastQC (version 0.11.5)