FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53518045
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC251250.023.2517859
GATCGGA272150.021.5725461
AGAGCGT289150.020.3294418
ATCGGAA300300.019.707342
TCGGAAG316600.018.7035263
CGGAAGA346100.017.3188514
AAGAGCG351450.016.9317447
CGCCGTA114350.011.41146945-49
GTCGCCG140600.09.43556440-44
GCCGTAT141550.09.42352145-49
TCGCCGT125100.09.21393345-49
CCGTATC151600.08.85619345-49
TGGTCGC151450.08.82660640-44
GAAGAGC713100.08.8121736
CGTATCA160800.08.38556245-49
GTGGTCG161350.08.34793140-44
GGTCGCC162500.08.33346540-44
GCGTCGT189300.07.08356410-14
CGTCGTG192750.06.994380510-14
TCTCGGT215650.06.420699635-39
Produced by FastQC (version 0.11.5)