FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51934970
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC227050.016.2189969
GATCGGA256200.014.4728191
CGCCGTA65850.014.40157755-59
AGAGCGT258900.014.3357798
ATCGGAA283900.013.1515172
TCGGAAG296550.012.6382613
TCGCCGT78950.012.19562455-59
AAGAGCG312550.012.060887
CGGAAGA315750.012.0075134
GTCGCCG90550.010.44112455-59
GCCGTAT95150.010.19542755-59
TGGTCGC109100.09.07674850-54
CGTATCA118150.08.43052760-64
GTGGTCG117350.08.42627450-54
CCGTATC107850.07.084711660-64
GGTCGCC110100.06.874977655-59
TCTCGGT158150.06.56406845-49
GAAGAGC676400.06.02320776
CGGTGGT178450.05.96367350-54
CTCGGTG178500.05.815726845-49
Produced by FastQC (version 0.11.5)