FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56551532
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA183100.016.8649921
ATCGGAA215450.014.5075242
TCGGAAG226050.014.0155083
CGGAAGA249150.012.5414674
TATGCCG89100.011.34227345-49
CTCGTAT101750.09.90365340-44
CGTATGC103150.09.75517945-49
TCTCGTA104150.09.29955640-44
ATGCCGT126700.07.930519645-49
GCCGTCT128150.07.569287350-54
ATCTCGT134550.07.133773340-44
CGTCTTC167950.06.319439450-54
CCGTCTT174800.05.91419450-54
AGAGCAC573250.05.8554628
ACACGTC181600.05.73262610-14
CGTCTGA176300.05.715806515-19
TCGTATG121000.05.58400245-49
GAGCACA624800.05.52307569
GAAGAGC638650.05.5056776
TGCCGTC125800.05.359444650-54
Produced by FastQC (version 0.11.5)