FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16144326
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35750.012.16781140-44
TATGCCG32500.012.09047145-49
GATCGGA89400.011.0070641
ATGCCGT40700.010.72332345-49
ATCGGAA95800.010.5949532
TCTCGTA35750.010.18040340-44
TCGGAAG100850.09.991943
CGGAAGA105250.09.5052284
CGTATGC35000.08.86549645-49
ATCTCGT44950.07.80643840-44
CGTCTTC55650.07.347668650-54
GCCGTCT44350.07.258134450-54
CCGTCTT57150.06.824984650-54
TCGTATG40050.06.62545740-44
TGCCGTC42550.06.16788645-49
AGATCGG92150.05.9003735145
AGAGCAC195500.05.78473338
GAAGAGC211900.05.43965866
GAGCACA212450.05.42557679
GTATGCC78700.05.39821545-49
Produced by FastQC (version 0.11.5)