FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58219513
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC49650.016.0602873
TATGCCG133050.015.14767545-49
GATCGGA393950.014.9397591
ATCGGAA435900.013.5534732
TCGGAAG445300.013.3324843
CTCGTAT151050.013.32308740-44
CGTATGC155800.012.7682945-49
CGGAAGA467700.012.7559364
TCTCGTA149700.012.44564640-44
CCGATCT69050.011.9679854
ATGCCGT174500.011.57448745-49
TTCCGAT72850.011.24420452
GCCGTCT182600.010.72849550-54
ATCTCGT185450.010.04644540-44
CGTCTTC219300.09.18433750-54
CCGTCTT221100.09.03086650-54
CTTCCGA99100.08.5793321
AGAGCAC771000.08.0397328
GAGCACA829800.07.52245479
GTATGCC291900.07.425969645-49
Produced by FastQC (version 0.11.5)