FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58219513
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA109850.017.55676555-59
TCGCCGT123800.015.53158755-59
GAGCGTC365150.015.1473199
GCCGTAT136900.014.1195155-59
GATCGGA421250.013.920661
AGAGCGT408100.013.8018868
GTCGCCG139950.013.26264155-59
ATCGGAA461600.012.611962
AGCGTCG306600.012.5546589
TGGTCGC159900.012.4134350-54
TCGGAAG470050.012.3995863
GTGGTCG170300.011.76603750-54
CGGAAGA496050.011.7496734
AAGAGCG490400.011.6928837
CGTATCA164200.011.39069660-64
CGGTGTA175650.011.25926135-39
ACCGGTG197500.09.96222735-39
CACCGGT199900.09.31313635-39
TCTCGGT222550.09.25756745-49
CCGGTGT219500.09.13547935-39
Produced by FastQC (version 0.11.5)