FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72345672
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT932380.12887847665579774TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA665100.023.396281
ATCGGAA696450.022.4361042
TCGGAAG704850.022.2363153
CGGAAGA723000.021.5878584
TATGCCG227450.017.52475445-49
TCTCGTA244750.015.61582340-44
CTCGTAT252450.015.54155940-44
ATGCCGT264250.014.90314445-49
AGAGCAC1079850.014.8633438
CGTATGC250850.014.80908345-49
GAGCACA1133250.014.1629679
GCCGTCT276500.013.39807650-54
GAAGAGC1203600.013.2990536
ATCTCGT286700.012.49140940-44
CGTCTTC327950.012.374907550-54
TGCCGTC271950.011.71885950-54
TCGTATG274000.011.5998745-49
CCGTCTT326500.011.51061350-54
AAGAGCA1568350.010.5018957
GGAAGAG1812900.09.1652565
Produced by FastQC (version 0.11.5)