FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33820127
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGCAAAATCTCGTAT569720.16845590201361454TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA325200.032.1288721
ATCGGAA333500.031.3707892
TCGGAAG338600.031.2772543
CGGAAGA351950.030.0085354
AGAGCAC522250.020.2231988
TATGCCG118000.019.08731845-49
GAGCACA555500.018.9094059
GAAGAGC572600.018.5714366
CGTATGC128650.016.70884545-49
CTCGTAT132650.016.6043540-44
TCTCGTA132450.016.5309640-44
ATGCCGT138300.015.89308545-49
GCCGTCT140100.015.25326450-54
TGCCGTC136650.015.01753850-54
TCGTATG141950.014.81253445-49
AAGAGCA753850.014.4233657
ATCTCGT150000.014.20076140-44
CGTCTTC162300.014.149199550-54
CCGTCTT164300.013.22024950-54
GGAAGAG852650.012.8880015
Produced by FastQC (version 0.11.5)