FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75347965
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA515200.024.9560931
ATCGGAA536750.024.1079182
TCGGAAG545100.023.5418663
CGGAAGA577950.022.4546954
TATGCCG184750.015.11525345-49
AGAGCAC976700.013.4566148
CTCGTAT210850.013.15498740-44
TCTCGTA207400.013.10116440-44
CGTATGC207800.012.95716445-49
GAGCACA1037050.012.7993129
GAAGAGC1088700.012.2321296
ATGCCGT227800.012.21419545-49
GCCGTCT236950.011.32062850-54
TCGTATG232600.011.1143845-49
TGCCGTC233050.010.93768150-54
ATCTCGT244150.010.75500940-44
CGTCTTC284550.010.07913450-54
CCGTCTT283750.09.54545250-54
AAGAGCA1483200.09.1154967
GGAAGAG1711100.08.2022135
Produced by FastQC (version 0.11.5)