FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45560411
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA102050.010.6129655-59
GAGCGTC370450.010.1363419
TCGCCGT115050.09.35074455-59
AGAGCGT407300.09.3082578
GCCGTAT122500.09.01877955-59
GATCGGA420950.08.7832611
ATCGGAA446000.08.4352472
TCGGAAG455950.08.2830293
AAGAGCG472350.08.0263637
GTCGCCG138200.07.889303755-59
CGGAAGA480700.07.84167534
CGTATCA143950.07.69481860-64
TGGTCGC148700.07.537556650-54
GTGGTCG157400.07.074871550-54
GCGTCGG43850.06.612762145
TCTCGGT206950.05.913524645-49
CCGTATC137900.05.66684360-64
AGCGTCG321700.05.40805639
CGGTGGT217000.05.39900550-54
GAAGAGC776200.05.2858386
Produced by FastQC (version 0.11.5)