FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75908264
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGGTTTGTGTAGATCT1441990.1899648238563327Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC615500.040.7362489
AGAGCGT677850.037.181738
GATCGGA740600.036.11711
ATCGGAA761400.034.985432
TCGGAAG768900.034.3848883
AAGAGCG763800.033.3868837
CGGAAGA796800.033.025694
CGCCGTA247500.022.19575755-59
TCGCCGT265750.020.45326855-59
GAAGAGC1303500.020.2317456
GTCGCCG287850.019.03908755-59
GCCGTAT287750.018.90462555-59
TGGTCGC313150.017.65439650-54
GTGGTCG326550.017.2185550-54
CGTATCA318200.017.02721860-64
CCGTATC308300.015.62236960-64
GGTCGCC317700.015.34723855-59
GACGGTT361050.015.18738730-34
TCTCGGT395950.014.41321845-49
GGAAGAG1920900.014.1668475
Produced by FastQC (version 0.11.5)