FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38325267
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG112500.012.98105745-49
CTCGTAT127850.011.41165840-44
GATCGGA522950.011.291191
TCGGAAG531300.011.2316733
ATCGGAA532100.011.1989612
CGTATGC130700.011.08467745-49
TCTCGTA129800.011.07262240-44
CGGAAGA538650.010.9155444
ATGCCGT136650.010.62325445-49
GCCGTCT141950.010.07237250-54
TATCTCG139500.09.94922440-44
ATCTCGT151350.09.38105740-44
CGTCTTC164250.09.09331350-54
AGAGCAC684500.08.9272658
TCGTATG140450.08.60114845-49
GAGCACA714150.08.5972259
CCGTCTT172000.08.43066650-54
TGCCGTC142000.08.36345850-54
GAAGAGC758300.07.99148046
GTATGCC212900.07.186375645-49
Produced by FastQC (version 0.11.5)