FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64474428
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA82250.010.7407581
AGCGTCG435550.09.9527319
AGCGGCG106500.09.5292249
GCGTCGG57000.09.159538145
GAGCGTC507350.08.659088
CGCCGTA136250.07.968184545-49
AGAGCGT557850.07.9661747
ATCGGAA646750.07.6199621
TCGGAAG655250.07.44610072
AAGAGCG654400.07.3890966
CGGAAGA674200.07.25738243
GCCGTAT163700.06.96851445-49
GTCGCCG181100.06.44280240-44
CCGTATC184400.06.304164445-49
GAGCGGC151600.06.2643918
CGTATCA187600.06.096187645-49
TGGTCGC202750.05.826316440-44
GGTCGCC209750.05.770079640-44
AGAGCGG168400.05.7255387
CGTCGTG402700.05.6000857145
Produced by FastQC (version 0.11.5)