FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005005357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70276297
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC517350.09.7825179
CGCCGTA148800.09.36351955-59
AGAGCGT580400.08.8322568
TCGCCGT169500.08.39108155-59
GATCGGA637700.08.0650831
ATCGGAA660350.07.86019232
GCCGTAT179600.07.84654655-59
TCGGAAG676050.07.7633833
AAGAGCG682650.07.65802537
CGGAAGA693050.07.58341264
GTCGCCG206050.06.972999655-59
CGTATCA208000.06.9561860-64
TGGTCGC212950.06.77467350-54
GTGGTCG232700.06.386609650-54
CGTCGTG414750.05.331763145
CCGTATC202550.05.25372660-64
AGATCGG650350.05.2397203145
GCGTCGG70650.05.2337794145
TCTCGGT301000.05.23102945-49
TCGTGTA411300.05.0591855145