FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005005373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005005373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47776825
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC301900.013.6384019
CGCCGTA78000.013.253098555-59
AGAGCGT339850.012.1794328
GATCGGA349700.011.9807831
ATCGGAA365150.011.4769652
TCGCCGT91150.011.30929255-59
TCGGAAG378550.011.0112583
CGGAAGA400350.010.5022054
GCCGTAT101950.010.43833755-59
AAGAGCG403750.010.3957897
GTCGCCG105550.09.76638555-59
CGTATCA120750.08.83709360-64
TGGTCGC120750.08.78956750-54
GTGGTCG132750.08.11517350-54
CCGTATC116800.07.08780960-64
GCGTCGG37100.06.6440134145
GGTCGCC125800.06.557739355-59
TCTCGGT172550.06.528681345-49
GAAGAGC712050.06.3426516
CTCGGTG194550.05.797860645-49
Produced by FastQC (version 0.11.5)